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In a similar fashion, female animal numbers from Pedigree A (3, 5, 7, 9, 11, 13 and 15) should only replace female positions in Pedigree B (18, 20, 22, 24, 26, 28 and 30). The I and C series, Health problems related to colour genes Changes have been made on almost every page, and information added about new alleles such as cryptic/atypical/harlequin merle, Northern domino, French Bulldog cocoa, Intensity, and more. In each pair, one is from the mother (egg) and one is from the father (sperm). With some breeds living as short as 7 years and as long as 18 years. Breeders should be aware that the inbreeding coefficient is a measurement of risk and does not guarantee that puppies produced will, or will not, have any inherited health conditions. We want to know the chances that a male patient with hemophilia will have a baby with this disorder. If you require further information on how to enter, please read below. An organism with two different alleles at a gene locus (one dominant and one recessive - Aa) has a heterozygous genotype. Dr. Matthew Breen is a professor of Genomics and the Oscar J. Fletcher Distinguished Professor of Comparative Oncology Genetics in the Dept. All the calculations are based on The American Kennel Club's database of adult dog weights and heights. If you have any difficulties then try switching to a different browser or using a PC/laptop. This means that the sex of offspring is determined by whether the sperm that fertilizes the egg carries an X or a Y chromosome. The Coat Color/Trait Calculator allows you to use results from dams and sires already tested in your account or you can manually create a hypothetical pairing by selecting the potential genotypes of the dam and sire (for up to 6 colors/traits). Link to Labgenvets page on Dog Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding, 2023 Labgenvet / Tous droits rservs / All Rights Reserved, Frequencies of genetic disease mutations by breed, Dog Genetics 3.0: Simple Genetic Diseases, Dog Genetics 4.0: Evolution, Breeds, Breeding strategies and Inbreeding, Dog Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, Dog Genetics 4.2: Pedigree based Inbreeding Coefficients of dog breeds as calculated and provided by The Kennel Club, for 2019, Veterinary Continuing Education Dog Genetics Demystified, Frequencies of Genetic Disease Mutations by Cat Breed, Cat Genetics 2.2: Glossary of Colour and Coat Genetics, Cat Genetics 3.0: Simple Genetic Diseases, Cat Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding, Cat Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, Horse Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding, Horse Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, CVMA Small Animal Practitioners Award 2022 for Labgenvet, Laboratory of Veterinary Genetics " For the genetic improvement of our domestic animals ", www.thekennelclub.org.uk/services/public/mateselect/, Dog Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding, Canine Disease Profile Primary Open Angle Glaucoma, Canine Disease Profile Multi-Drug Resistance (MDR1), Canine Disease Profile Degenerative Myelopathy (DM), Canine Disease Profile PRA-Type-1 Papillons, Cat Genetic Profile Hypertrophic Cardiomyopathy (HMC), Cat Genetic Profile Feline Blood Groups. Although the CoI is not a guarantee of health, it is a measure of risk with a higher CoI suggesting a higher risk. List of alleles for reference, Agouti, sable, tan points and saddles It also features a new advanced colour and coat genetics calculator that I've helped to design, so you can get detailed predictions for litters before you breed. Dog Age Calculator | Compare Dog Years vs Human Years - Dogbreedo Each CoI calculation is accompanied by a breed average. Figure 1. Each of the 78 rod-shaped structures are separate chromosomes. At Paw Print Genetics, we are always happy to answer those questions and help our customers understand their results and what the outcome might be for a given breeding pair. It must be stated that an inbreeding coefficient is just one tool amongst many others available to breeders to help them in making breeding decisions. For the intrepid souls wishing a more thorough discussion of the genetics of white spotting, I refer you to more comprehensive reviews: http://homepage.usask.ca/~schmutz/dogspots.html. These new tools allow breeders to calculate the possible outcomes from potential breeding pairs based on their genetic test results. This pattern can be the banded hair pattern (light shaft, dark tip) seen in German Shepherds and wolves, or in the black and tan pattern of yellow pigment seen in many dog breeds. Changes have been made on almost every page, and information added about new alleles such as cryptic/atypical/harlequin merle, Northern domino, French Bulldog cocoa, Intensity Redbubble Many interesting points have been left out, including ticked patterns, progressive greying, the genetics of short, long and curly hair, among other topics. Since all allele combinations are equally likely to occur, a Punnett Square predicts the probability of a cross producing each genotype. Including body part diagram, Masks, recessive red and domino The K and A series, Oddities Chromosomes have thousands of genes with DNA-encoded traits, and each gene has allele pairs. Let's say we need to know the probability that our patients' baby will inherit a genetic disorder called cystic fibrosis. 438(7069): p. 803-19. If a number of breeding possibilities are available that will reduce the average inbreeding coefficient within the offspring compared to the breed average, then ideally the breeding that will result in the lowest inbreeding coefficient while still maintaining the desired traits for the breed is recommended. Bakia coat color calculator. Your breeding decisions should always be well balanced and take into consideration the qualities and compatibility of both the sire and dam that you are considering. The CoI calculator gives you a percentage score. It is important to realize that a single hair can be all black, all yellow, or yellow with a black band at the tip. The results table shows the expected percentage of dogs in the litter with each possible genotype and a brief interpretation. Inbreeding Calculator Pedigree A represents the pedigree of the first parent (father or mother). Second situation: only one parent is a carrier. The amount of DNA per chromosome was determined to range from approximately 126 million nucleotides for chromosome 1, to just 27 million for chromosome 38. In all mammals, there are two chromosomes, X and Y, that determine the sex of an individual. A Punnett Square* shows the genotype*s two individuals can produce when crossed. Every gene has two versions, called alleles. Dog Color Genetics 101 (With Breeding Chart!) - Hepper (Autosomal inheritance means that described genes are located on regular chromosomes [1-22], and not sex chromosomes [X,Y]). Dog Genetics 2.0: Colours | Laboratoire de gntique vtrinaire The Coat Color/Trait Calculator can be used by the general public and both the Coat Color/Trait and Disease Calculators can be used by Paw Print Genetics customers specifically for their dogs that have results from PPG testing. To find possible genotypes locate different combinations of alleles -, Based on the possible genotypes, you can assess the phenotypes. Both parents are healthy, but they still may be carries since the disorder is inherited in an autosomal recessive manner. Agouti: A/A A/a a/a What if the potential dam and sire are both carriers of a genetic disease? Locus E, also known as Extension, affects black pigment production. Let's look at the genetic table below. The allele combinations along the top and sides become labels for rows and columns within the square. Colour is produced by special chemicals called pigment. In some breeds, Locus D can be associated with alopecia or loss of hair, probably due to another as yet unidentified gene acting in combination with d/d. However, all of his daughters will be carriers, and may transfer the disease to the next generation. Recently, the gene involved in the S locus as well as four separate mutations associated with white spotting were identified. breeds and their close relatives. Change the ancestor numbers in Pedigree B (ancestors 16 to 30) to correspond to common ancestors in Pedigree A (ancestors 1 to 15). We hope our customers will find these calculators useful as they make preparations for future breedings. Unlike pedigree based COI calculations, genetic COI evaluates the actual pieces of DNA in your dog to identify which proportion traces back to inbreeding. our genetic system is in duplicate (see Labgenvets page on Dog Genetics 1.0: The Basics). For a given gene, an individual dog can have two copies that are the same (either two dominant or two recessive copies), or one copy that is dominant and one copy that is recessive. The colour modifying genes do not make the colour pigments, but they do influence the shade of the colour, usually by reducing (diluting) colour intensity. Metaphase chromosomes of the domestic dog as they appear when stained with a blue fluorescent dye that allow them to be seen with a high-powered microscope. A short genetics quiz! Sex cells (sperm and eggs) have only one strand of chromosomes. Dog Genetics 3.0: Simple Genetic Diseases. Pedigree A represents the pedigree of the first parent (father or mother). This brief review of the genetics of coloration in the dog should be considered a starting point, designed to simplify what can become quite complex. Simply, fill in all the relevant questions and click on the "Calculate Ideal Dog" button. Heterozygous - We use it where one allele is recessive (a), and the other is dominant (A). Each of a dog's cells (except sex cells) contains two strands of chromosomes, one from the father and one from the mother. Reinitialize the Calculator by pressing the Reset button. If you already know you blood type why don't you check who you could possibly donate it to?Try using our Blood donor calculator. What's the difference? The CoI calculator gives you a percentage score. Langford,The DAPI banded karyotype of the domestic dog (Canis familiaris) generated using chromosome-specific paint probes. More broadly, genetics covers the study of genes, how they are organized in cells, how they are regulated, and how they interact with each other and the environment. All of his sons will be completely free of the disease. The E series, Great Danes Look at the result of the Punnett square. Homozygous genotype signifies the presence of two identical alleles (both normal or identically mutated - AA or aa). On average, 75% of children born to these parents will be healthy: out of those 2/3 will be carriers, and 1/3 will inherit no cystic fibrosis alleles. For two traits, an individual can produce 4 allele combinations (2^2). For more information on Inbreeding and the Inbreeding Calculator, go to: Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding. Locus E also has a dominant version (EM), which gives the Black Mask pattern of coloration in the face that is seen in many dog breeds. Only males have the Y chromosome, and all female eggs have an X chromosome. This allele is picked at random, so each of the two alleles has an equal chance ( 50%) of being passed on. The Agouti gene, found at Locus A is the master gene for yellow pigment production. Dog Coat Colour Genetics ** 20/05/2020: website fully updated with lots of new research and corrections. This article is the first in a two-part series. dog genetics calculator Menu fatal shooting in los angeles today. The ChromaGene test will determine which one of the genotypes listed below your Labrador Retriever is: Coat Color Inheritance Chart for the Labrador Retriever ChromaGene Type During that era, humanity had no microscopes, complex scientific technology, or the slightest concept of genes. Embark's genetic COI assesses over 230,000 markers and can detect inbreeding in far more past . Punnet squares and a big Labrador case study, Dilution (blue and isabella) Figure 2. The animal that you are considering as a potential breeding partner is represented by animal 16 in the lower parental pedigree (Pedigree B, with animals 16 to 30). Guy Labb, DVM. The probability of a cross producing a genotype in any box is 1 in 16. In general, the lower the percentage, the lower the degree of inbreeding. Evidently, for ancestors in common between the two pedigrees, male animal numbers from Pedigree A (2, 4, 6, 8, 10, 12 and 14) should only replace male positions in Pedigree B (17, 19, 21, 23, 25, 27 and 29). The Coat Color/Trait Calculator first generates a table of the possible outcomes from a hypothetical breeding between dogs based on the selected trait genotypes of the potential dam and sire. What will they look like? In practice it is recommended to chose crosses that will result in offspring that have reduced coefficients of inbreeding compared to the average of the breed in question. The Needs and Activities of the Dog. There is a 25% chance (1/4) of giving birth to a child with cystic fibrosis. Cryptic, atypical, and harlequin merle, Merle modifiers (Great Dane harlequin) Autosomal alles - homozygous or heterozygous? dogs with relatives in common. If you purchase a product through this article, we may receive a portion of the sale. It is the interplay between the expression of the genes responsible for these two basic pigments that give the dog its basic coloration. AKC is a participant in affiliate advertising programs designed to provide a means for sites to earn advertising fees by advertising and linking to akc.org. Chromosome Research, 1999. There are children with cystic fibrosis in both of families. Moreover, our Punnet square maker allows you to calculate the probability that a rare, recessive genetic disease will be inherited. The probability of a cross producing a genotype in any box is 1 in 16. Both parents are heterozygous, and one allele for each trait exhibits complete dominance*. Adding more traits increases the size of the Punnett Square. One combination produces a double recessive offspring. One pair of genes determines the animal's sex, and the remaining ones affect everything else that makes the dog unique. On the other hand, an elevated inbreeding coefficient will increase the chances that desirable traits associated with the breed in question will be fixed. The banding patterns are the same for each pair, allowing the pairs to be identified. Thus, the inbreeding coefficient can be viewed as a compromise. Over the course of a series of short articles, we will cover the basics of what DNA is and how it is organized in cells, how cells divide and pass genetic information to the next generation, the laws of inheritance, the molecular basis of disease, how purebred dogs can help advance genetic research, and how genetic variation (inherited and sporadic) impacts disease. This is in contrast to the Dominant Yellow seen with the Agouti locus, and can be the source of some confusion. Nature, 2005. We use capital letters for dominant alleles (A), and lowercase for recessive alleles (a). Have you ever wondered what the outcome of a particular breeding might be? Genotype and phenotype probabilities with a monohybrid cross, Genotype and phenotype probability questions, Test sensitivity - specificity calculator, How earthquakes show us the inside of the Earth, Surface currents, the Ekman spiral, and Ekman transport. Select the dam and sire and the specific disease tests (up to 8) that you want to include in your calculation. Pedigree B represents the pedigree of the second parent (mother or father). It is the actual DNA sequence where differences between breeds are detected. The letters in the manual represent the nucleotides of the DNA, the words represent the genes, and the chapters represent the chromosomes. They found similar processes occurring in a 7-week-old puppy and a 9-month-old human baby. The research team also used the rate of the methylation changes in dogs to match it to the human epigenetic clock, although the resulting dog age conversion is a bit more complex than "multiply by seven." The new formula, which applies to dogs older than one, says that a canine's human age roughly equals 16 ln (dog age) + 31. Hemophilia is a rare genetic, X-linked disease. The two sex chromosomes (X and Y) are labeled, indicating that this cell is from a male. The Agouti gene does double duty, acting as a master gene for yellow pigment formation, but also involved in colour pattern formation. Select the dam and sire and the specific disease tests (up to 8) that you want to include in your calculation. What kind of results will the calculators provide? For example, more than ten genes influence eye color! of Molecular Biomedical Sciences at the NC State University College of Veterinary Medicine. To calculate the inbreeding coefficient for a given animal, the paternal pedigree (Pedigree A, representing ancestors 1 to 15) is compared to the maternal pedigree (Pedigree B, representing ancestors 16 to 30). Dog of coat color DNA calculator - YouTube When mammals such as dogs breed, each parent passes on just one allele from each of its loci. This number helps you compare any potential matings to the average of the breeds current population and puts that CoI into perspective. Copyright 2013-2023 All Rights Reserved. reducing litter sizes and fertility.You can find out more about inbreeding, genetics and DNA testing in a number of films available onThe Kennel Club Academy. A second variation at the K locus is responsible for the Brindle coloration (Kbr) seen in some breeds such as the Boxer. Inside all animal cells (with the exception of red blood cells) is a nucleus that contains the genetic material, deoxyribonucleic acid (DNA). Genotype describes hidden genetic properties of a trait. A two-trait Punnett Square has 16 boxes. Traits are inherited through genes, the memory banks of the cell. For the Color/Trait Calculator, you can also manually select potential genotypes for a hypothetical dam and sire to see the potential outcomes. n = the number of pathways (number of generations) between a common ancestor and the individual in question. Paw Print Genetics - New Coat Color/Trait and Disease Probability Our Punnett square maker works on autosomal alleles (chromosomes 1-22), but it can be used for other things. It is important to note that ancestors in common within a parental pedigree are not utilized in the calculation, only ancestors in common between the parental pedigrees. The DNA test for b and e allows breeders the choice of producing dogs who are chocolate or yellow, or the opportunity of breeding away from these colors. To allow all the DNA from a single cell to fit inside a nucleus,a special protein scaffold allows the DNA to wind tightly into bundles. The calculator is based on four generations of ancestors (parents, grandparents, great-grand parents and great-great-grandparents), including 30 ancestors in total, using a simplified version of the formula proposed by Sewell Wright for calculating inbreeding coefficients: In order to further simplify the process, a number is used to designate a specific animal within the pedigree, rather than the complete name of the animal which can be cumbersome. This further simplification may result in an underestimation of the absolute value of the inbreeding coefficient calculated for a given animal, however this should not interfere with conclusions made when comparing pedigrees and potential mating partners when performing virtual crosses. Our CoI calculators use all available, electronically held, pedigree information and they do not limit the number of generations used. Interestingly, white is not a colour per se, but is rather a lack of colour. Step 1: Sire Color Select Sire starting color Blank Slate Click on allele combination to edit combinations. One allele per row must be selected. If your dog was a human, how old would he be? Fill the first column and row with the parent's alleles. Let's think about X-linked diseases - disorders that are inherited only via the female line of the family. 7(5): p. 401-6. Calculation accuracy of the offspring color possibilities and probabilities can be greatly increased when providing the color genetics of the sire and dam. We will see the function of single genes, the passage of genes to the next generation, and importantly, the interaction of genes with each other to give the combinations of colour traits seen between and within the dog breeds. Making a simple 1 trait gene chart is extremely easy! As well as considering the implications of a dogs inbreeding coefficient, there are other equally important factors to consider when deciding whether two dogs should be mated together, such as temperament, genetic diversity, conformation, other available health test results, the general health of the dogs etc. per week while larger breeds will gain around 2 lb 5 oz. The final questions will help you find your ideal type of dog, thanks to the Dog Breed Calculator, are centred around the activities you would like your dog to be able to do. Because the Disease Calculator only works with dams and sires in your account who have had testing completed at Paw Print Genetics, it can only be reached by logging in to your Paw Print Genetics account and selecting it from the My Account dropdown. The average life expectancy of dogs is 10-13 years. Why does it matter? Incestuous crosses resulting in offspring with coefficients of inbreeding above 12.5% should not be performed; these include parent-offspring, brother-sister, grandparent-grandchild, half-brother-half-sister matings. It comes as handy if you want to calculate the genotypic ratio, the phenotypic ratio, or if you're looking for a simple, ready-to-go, dominant and recessive traits chart. Dog Breeder Pro is a web-based software solution for dog breeders, with a range of useful features to help you keep track of pedigrees, waiting lists, test results and more. published scientific studies). The shadow dog is the total of the recessive genes that are unseen, but are ready to become apparent in future generations. Dilution is a recessive trait that will modify normal black pigmentation to give a grey colour (d/d) called either Blue, Grey or Slate depending on the breed. The precise order of the nucleotides that make up the canine genome sequence was determined in 2005, revealing a total of approximately 2.4 billion nucleotides [2]. A genome can be thought of as an instructional manual. References: More comprehensive and in depth coverage on the topic of dog colour genetics can be found with these websites: http://homepage.usask.ca/~schmutz/dogcolors.html, 2023 Labgenvet / Tous droits rservs / All Rights Reserved, Frequencies of genetic disease mutations by breed, Dog Genetics 3.0: Simple Genetic Diseases, Dog Genetics 4.0: Evolution, Breeds, Breeding strategies and Inbreeding, Dog Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, Dog Genetics 4.2: Pedigree based Inbreeding Coefficients of dog breeds as calculated and provided by The Kennel Club, for 2019, Veterinary Continuing Education Dog Genetics Demystified, Frequencies of Genetic Disease Mutations by Cat Breed, Cat Genetics 2.2: Glossary of Colour and Coat Genetics, Cat Genetics 3.0: Simple Genetic Diseases, Cat Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding, Cat Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, Horse Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding, Horse Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, CVMA Small Animal Practitioners Award 2022 for Labgenvet, Laboratory of Veterinary Genetics "For the genetic improvement of our domestic animals", Canine Disease Profile Primary Open Angle Glaucoma, Canine Disease Profile Degenerative Myelopathy (DM), Canine Disease Profile Multi-Drug Resistance (MDR1), Canine Disease Profile PRA-Type-1 Papillons, Cat Genetic Profile Hypertrophic Cardiomyopathy (HMC), Cat Genetic Profile Feline Blood Groups. Well, calculating your dog's age in human years isn't that simple. The two basic pigments that contribute to coloration are black and yellow pigments. Do not forget to reinitialize the calculator between calculations by pressing the Reset button. With simple experiments and insightful observations, he was able to draw conclusions that are useful up to this day - it's no wonder he's called the Father of genetics. The Coat Color/Trait Calculator can also be reached from the Coat Color and Trait Testing page of the Paw Print Genetics website: https://www.pawprintgenetics.com/products/traits/. This average is shown to you each time you use our CoI calculators. Course topics include introduction to breeding, genetics, anatomy, whelping and nutrition. There are types of alleles which are superior to the other types (dominant alleles); The chance either allele will be inherited is equal; and, Xd - X chromosome with Hemophilia gene; and. This is displayed in the results table and bargraph below: Adding results for more diseases (including those with more complicated genetic inheritance) increases the complexity of the calculation. Puppy Size Calculator | How Big Will My Dog Get? | YourPaws (When the percentages get confusing - try the percentage tool.). AKC actively advocates for responsible dog ownership and is dedicated to advancing dog sports. A key component of DNA is the nucleotide. You can find out more about the impact of inbreeding in our, You can find out more about inbreeding, genetics and DNA testing in a number of films available on, 0% = a dog with two apparently unrelated parents (based on all available pedigree information), 12.5% = the genetic equivalent of a dog produced from a grandfather to granddaughter mating, or the mating of a half-brother/sister, 25% = the genetic equivalent of a dog produced from a father to daughter mating, or the mating of full-brother/sister, More than 25% - inbreeding is accumulative, so if it has occurred to a significant degree over several generations, the inbreeding coefficient may exceed 25%, When using our CoI calculators always check the about this calculation section to see how many generations have been used for calculation, If this indicates that only the first three generations are fully complete, you may wish to use the CoI result with care, If additional pedigree information or inbreeding resources are available to you, then you may wish to consult these. Genetics of Dog Breeding | Dog Breeding Information 2023 Labgenvet / Tous droits rservs / All Rights Reserved, Frequencies of genetic disease mutations by breed, Dog Genetics 3.0: Simple Genetic Diseases, Dog Genetics 4.0: Evolution, Breeds, Breeding strategies and Inbreeding, Dog Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, Dog Genetics 4.2: Pedigree based Inbreeding Coefficients of dog breeds as calculated and provided by The Kennel Club, for 2019, Veterinary Continuing Education Dog Genetics Demystified, Frequencies of Genetic Disease Mutations by Cat Breed, Cat Genetics 2.2: Glossary of Colour and Coat Genetics, Cat Genetics 3.0: Simple Genetic Diseases, Cat Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding, Cat Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, Horse Genetics 4.0: Evolution, Breeds, Breeding Strategies and Inbreeding, Horse Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation, CVMA Small Animal Practitioners Award 2022 for Labgenvet, Laboratory of Veterinary Genetics " For the genetic improvement of our domestic animals ". Training isn't just for pets - here's your chance to learn too. There isn't a perfect formula A dog age . It includes a range of mixed breed categories, which reflect the approximate adult size of the dog. Dog Breed Calculator | Types of Dog Calculator | Choosing a Pet Founded in 1884, the not-for-profit AKC is the recognized and trusted expert in breed, health, and training information for all dogs. The nuclei of dog cells contain important genetic data. The numbers in the lower parental pedigree are changed to reflect common ancestors with the pedigree of your animal. Just as a recipe can be used to make a dish of food, a gene can be used to make a protein, a building block . To put your result into perspective: 0% = a dog with two apparently unrelated parents (based on all available pedigree information) The C Locus is a master gene that controls the formation of both black and yellow pigments. The Disease Calculator automates these complex calculations and presents the results of a prospective breeding in a simple and easily understandable format. Brown is a recessive trait that will lighten the normal black pigment (B) to give a brown colour (b/b). Independent assortment typically means the genes are on different chromosome*s. If the genes for the two traits are on the same chromosome, alleles for each trait will always appear in the same combinations (ignoring recombination). Traits are unitary (red color vs. yellow color); There are two versions of every gene (now we call them. Genetics 4.1: Inbreeding Calculator, Detailed Instructions and Interpretation. Why Have I Been Sent A Depop Verification Code,
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